Protocol for genome-wide 5’RNA identification

We jut got published a new paper describing alternative methods for the genome-wide identification of 5’RNA ends. In this paper we do not only describe in detail our previous 5PSeq approach, but also new methods to identified alternative 5’RNA modifications. In particular we provide alternatives to use of the recently discontinued Tobacco Acid Pyrophosphatase.

Genome-wide quantification of 5′-phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics. Pelechano V, Wei W, Steinmetz LM. Nat Protoc. 2016 Feb;11(2):359-76.