crosstalk between ribosome biogenesis and transcription elongation

Posted on June 22, 2017June 23, 2017 by vicent

Our collaborators from Sebastian Chavez lab (Seville) have just published a new paper showing the crosstalk between ribosome biogenesis and transcription elongation. Glad to have contributed to this story. The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation. Fernando Gómez-Herreros Thanasis Margaritis Olga Rodríguez-Galán Vicent Pelechano Victoria Begley Gonzalo Millán-Zambrano Macarena[…]

Using 5PSeq to unravel eIF5A dependent ribosome stalling

Posted on June 3, 2017June 23, 2017 by vicent

We have just published our first work 100% performed in our new lab at SciLifeLab-Karolinska Institutet. It has been a great collaboration with Dr. Paula Alepuz from the University of Valencia (Spain) eIF5A facilitates translation termination globally and promotes the elongation of many non polyproline-specific tripeptide sequences. Pelechano V# and Alepuz P#. Nucleic Acids Res. 2017 May 26. doi:[…]

A global genetic interaction network

Posted on October 4, 2016June 23, 2017 by vicent

Amazing work from our collaborators from Boone, Andrews and Myers Lab. A landmark in genetics that for sure will pave the way for many future discoveries. M. Costanzo*, B. VanderSluis*, E. N. Koch*, A. Baryshnikova*, C. Pons*, G. Tan, et al. A global genetic interaction network maps a wiring diagram of cellular function. Science, 2016;[…]

Principes of eukaryotic RNA metabolism

Posted on July 26, 2016June 23, 2017 by vicent

We want to congratulate our collaborators from Sandelin and Jensen Lab for leading this great paper in Nature Genetics. This is a great example of how to combine different genome-wide methodologies to improve our understanding of the transcriptome organization. Proud of our contribution to this work. Principles for RNA metabolism and alternative transcription initiation within[…]

Interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation

Posted on June 26, 2016June 23, 2017 by vicent

Great collaboration with the Akhtar group. Nice to see this nice paper out. Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation. Chlamydas et al. Nature Structural & Molecular Biology. 2016 23, 580–589 doi:10.1038/nsmb.3233

Protocol for genome-wide 5’RNA identification

Posted on February 7, 2016June 23, 2017 by vicent

We jut got published a new paper describing alternative methods for the genome-wide identification of 5’RNA ends. In this paper we do not only describe in detail our previous 5PSeq approach, but also new methods to identified alternative 5’RNA modifications. In particular we provide alternatives to use of the recently discontinued Tobacco Acid Pyrophosphatase. Genome-wide[…]