Rapid colorimetric detection of Covid19

Happy to share our collaboration for the rapid colorimetric detection of Covid19 (iLACO).

Work pushed in record time by Xiushan Yin and his team at Shenyang. 20 minutes, LAMP based and just needs a water bath. Here the preprint and instructions disq.us/t/3mfefwc

iLACO

Update (13 March 2020):

As some people requested please find here the detailed instructions. The oligo sequences can be found in the preprint above. This info is that you can see in the link is the one enclosed in the humanitarismos help  of +20.000 diagnosis kits that Xiushan Yin and his team at Biotech (http://www.bio2k.cn , only in Chinese sorry for the moment. Use Chrome) have been donating to many countries (Pakistan, Eritrea…).

Colorimetric And Isothermal Detection Kit For COVID-19 Coronavirus

Additionally, here also an optimized qPCR approach for the same objective.

The Instructions of Real Time PCR Detection Kit

 

 

Update (21 March 2020):

Potential to mix with HUDSON to avoid RNA extraction, although decreases a bit sensitivity.

HUDSON (heating unpurified diagnostic samples to obliterate nucleases)
For the HUDSON protocol, cell culture supernatants were harvested, placed on ice, and mixed with tris(2-carboxyethyl)phosphine (TCEP, Thermo Fisher Scientific, used at 100 mM final concentration) and EDTA (Thermo Fisher Scientific, 1 mM final concentration). HUDSON mixtures were then incubated at 50C for 5 min, followed by 95C for 5 min to inactivate nucleases and viral particles respectively. Heat inactivated products were not diluted, but HUDSON products were diluted 1:10 with nuclease-free water prior to RT-qPCR. RT-qPCR was performed using the Power SYBR. It comes originally from this paper https://www.ncbi.nlm.nih.gov/pubmed/29700266